Part B-Dec 2012

CSIR-UGC National Eligibility Test (NET) for Junior Research Fellowship and Lecturer-ship

Part B


1. People suffering from glucose-6-phosphate dehydrogenase deficiency are found to be resistant to which of the following pathogens ?
a. Leishmania                b. Plasmodium
c. Fungus                        d. Bacteria
Ans. 2
Expln.  – G6PD deficiency is an X-linked recessive hereditary disease characterized by low levels of glucose-6-phosphate dehydrogenase which is involved in HMP pathway. G6PD catalyzes the oxidation of  Glucose-6-phosphate to 6-phosphogluconolactone. This shunt generates NADH and as a result reduced glutathione(GSH) is produced which is necessary for the reduction of H2O2. Hydrogen peroxide is a strong oxidant that will degrade the RBC and cause hemolysis if it is not reduced.
When a plasmodium enter the RBC, it oxidizes NADPH from the HMP pathway for its metabolism. Thus reduced glutathione(GSH) is not produced. Deficiency of GSH is more severe in G6PD deficient individuals, leading to peroxide-induced hemolysis which curtails the development of plasmodium.
Note: Relation between Favism and G6PDsee the simple instant notes.
2. For which of the following purpose, polygalacturonase antisense RNA is used.

1. Viral resistance                 2. Seed setting
3. Fruit ripening                    4.Herbicide resistance
Ans – 3

3. The proteins Bcl-2 and Bax are

1. Anti-apoptic and pro-apoptic        2. Pro-apoptic and anti-apoptic
3. Both pro-apoptic                            4. Both anti-apoptic
Ans- 1     Expln. :   Bcl-2(B-cell lymphoma 2) is the founding member of the Bcl-2 family of apoptosis regulator proteins encoded by the BCL2 gene. These proteins govern mitochondrial outer membrane permeabilization and can be either pro-apoptotic (Bax, BAD, etc) or anti-apoptotic (Bcl-2 proper). Bax (Bcl-2 associated x protein) is thus a protein of the Bcl2 gene family.

4. In which cancer treatment erb-B antibodies are used ?

1. Breast              2. Oral
3. Prostrate         4. Lung