CSIR-UGC National Eligibility Test (NET) for Junior Research Fellowship and Lecturer-ship
PREVIOUS SOLVED QUESTIONS – 2014 June CSIR – NET EXAM
This time CSIR does not allow candidates to carry questions with them. We have collected maximum questions from our candidates (memory based).
1. acetylated proteins
2. glycosylated proteins
4. nascent proteins
Expln:- Free ribosomes produce proteins utilized within the cell, while the membrane bound ribosomes produce proteins that are transported out of the cell for secretion, the lysosomes and the membrane formation. Ribosomes are attached to the endoplasmic reticulum through two transmembrane
2. Methylene blue
4. Crystal violet
1. pre-replicative complex can only form in G1 and replication can only be initiated when pre-replication complex is disassembled at the beginning of S-phase.
2. replication can only be initiated when pre-replication complex is intact
3. replication can only be initiated when unphosphorylated Rb is present
4. pre-replicative complex can only form in S-phase
Expln:- A pre-replication complex (pre-RC) is a protein complex that forms at the origin of replication during the initiation step of DNA replication.
In bacteria, the main component of the pre-RC is DnaA. The archaeal pre-RC is very different from the bacterial pre-RC and can serve as a simplified model of the eukaryotic pre-RC. It is composed of a single origin recognition complex (ORC) protein, Cdc6, and a homohexamer of the mini chromosome maintenance (MCM) protein.
1. of their higher magnification
2. the lenses used are of much higher quality
3. of very short wave length of electrons
4. the images are viewed on screen rather than directly using an eye-piece or occular lens
1. gene activator proteins
2. Specific promoter sequence
3. phosphorylation of histone acetylase
4. dephosphorylation of chromatin remodeling complexes
Expln:- In eukaryotes, DNA is tightly wound into a complex called chromatin. Chromatin not only serves as a way to condense DNA within the cellular nucleus, but also a way to control how that DNA is used. Specific genes are not expressed unless they can be accessed by RNA polymerases and proteins known as transcription factors. Therefore, a cell’s chromatin must â€œopenâ€ in order for gene expression to take place. This process of ‘opening’ is called chromatin remodeling.
The amount of binding between DNA and histones can be altered by a process called acetylation. The addition of the acetyl group to positively charged amino acids such as lysine removes the charge and reduces the affinity with negatively charged DNA. When the histones are deacetylated, the positive charges are restored and the DNA binds more tightly to the histone.
Specific DNA’s binding transcription factors recruit histone acetylases and deacetylases to promoters to activate or repress transcription.
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