CSIR-UGC National Eligibility Test (NET) for Junior Research Fellowship and Lecturer-ship
Some Important Questions Collected from the Memory of Students and Probable Questions and Answers
Previous Year Questions & Answers – Part C Dec. 2016
A.For a reaction to occur spontaneously the free energy change must be negative
B.The interaction between two nitrogen molecules in the gaseous state is predominantly electrostatic
C.By knowing bond energies, it is possible to deduce whether the bond is covalent bond or hydrogen bond
D.Hydrophobic interactions are not important in a folded globular protein.
Pick the combination with ALL WRONG statements.
1. A and B 2. B and C
3. C and D 4. B and D
Expln:- Since nitrogen is apolar, no there is no electrostatic interaction. Hydrophobic interactions are responsible for proper folding.
Which of the following treatments best represents the results shown in the graph ?
1.a = ammonium sulphate, b = glycerol, c = pH 6.4, d = CaCl2
2.a = CaCl2, b = glycerol, c = ammonium sulphate, d = pH 6.4
3.a = pH 6.4, b = CaCl2, c = ammonium sulphate, d = glycerol
4.a = CaCl2, b = pH 6.4, c = glycerol, d = ammonium sulphate
Expln:- Protein near their isoelectric point (Pi) value gets precipitated, as pH 6.4 is very near to its PI value so we will get minimum protein in the supernatant, which is either b or d. Ammonium sulphate also precipitates the proteins, so minimum protein in the supernatant. CaCl2 and glycerol will help in the solubilization they max protein will be present in the supernatant.
1.All N atoms, C4 and C5 are from aspartic acid
2.NI is from Aspartic acid; N3 from N9 are from Glutamine side-chain; N7, C4 and C5 are from Glycine.
3.NI is from Aspartic acid; N3 from Glutamine side-chain; N9 from N attached to Cα of Glutamine ; N7, C4 and C5 are from Glycine
4. NI is from Glutamine; N3 from Glutamine side-chain; N9 from N attached to Cα of Glutamine; N7, C4 and C5 are from Glycine
Expln:- Nitrogen N1 from aspartic acid, N3 and N9 from glutamine and N7, C4 and C5 comes from glycine.
More Questions & Answers Refer Simple Instant Notes